Principle of SNAP/CLIP-labeling and labeling protocols. (a) SNAP-and
Download scientific diagram | Principle of SNAP/CLIP-labeling and labeling protocols. (a) SNAP-and CLIP-tag fused to the protein of interest bind benzylguanine-or benzylcytosine-substrates, respectively. There are various cell-permeable fluorescent substrates and a non-fluorescent substrate available. (b) For labeling of the whole fusion protein pool cells are incubated with a fluorescent substrate, washed and fixed/imaged. (c) For age-dependent labelings cells are first incubated with the non-fluorescent blocker followed by labeling of the newly-synthesized fusion protein with a fluorescent substrate. To omit continuous labeling with the first substrate additional blocking/ labeling steps can be added. from publication: A Global Approach for Quantitative Super Resolution and Electron Microscopy on Cryo and Epoxy Sections Using Self-labeling Protein Tags | Correlative light and electron microscopy (CLEM) is a powerful approach to investigate the molecular ultrastructure of labeled cell compartments. However, quantitative CLEM studies are rare, mainly due to small sample sizes and the sensitivity of fluorescent proteins to | Protein Tagging, Electron Microscopy and Correlative Light Electron Microscopy | ResearchGate, the professional network for scientists.
Protein Labeling
PDF) Fluorescent Labeling of SNAP-Tagged Proteins in Cells
A Global Approach for Quantitative Super Resolution and Electron Microscopy on Cryo and Epoxy Sections Using Self-labeling Protein Tags
Characterization of SNAP-and CLIP-tag labeling kinetics. (A) Comparison
Design of a palette of SNAP-tag mimics of fluorescent proteins and their use as cell reporters
IJMS, Free Full-Text
Cellular Analysis (SNAP-Tag) - New England Biolabs GmbH
Ultrafast tissue staining with chemical tags
Protein Labeling Snap Clip
SNAP-tag and CLIP-tag overview
Injection molding design fundamentals: Snap-fits for plastic parts
Selective Cross-Linking of Interacting Proteins Using Self-Labeling Tags
Development of a Split SNAP-CLIP Double Labeling System for Tracking Proteins Following Dissociation from Protein–Protein Complexes in Living Cells
Evaluation of Fluorophores to Label SNAP-Tag Fused Proteins for Multicolor Single-Molecule Tracking Microscopy in Live Cells: Biophysical Journal
